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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Myeloperoxidase Antibody for FACS / MPO Flow Cytometry Antibody (RQ7843)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7843 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P05164 |
| Applications | Western Blot : 0.5-1ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This Myeloperoxidase antibody is available for research use only. |
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Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme encoded by the MPO gene and is abundantly expressed in neutrophils and other cells of the myeloid lineage. MPO is localized to azurophilic granules where it contributes to host defense by catalyzing the formation of reactive oxidant species during the respiratory burst of activated phagocytes. Because MPO expression is highly enriched in granulocytes and early myeloid precursors, the protein has become a widely used marker for identifying and analyzing myeloid cell populations in immunological and hematological research. Myeloperoxidase Antibody for FACS enables sensitive detection of Myeloperoxidase / MPO at the single-cell level using flow cytometry, supporting quantitative analysis of neutrophils and other MPO-positive cells within heterogeneous samples.
Flow cytometry analysis with a Myeloperoxidase Antibody for FACS allows researchers to rapidly evaluate intracellular MPO expression across thousands of individual cells in suspension. Following fixation and permeabilization, MPO-positive cells can be clearly distinguished based on fluorescence intensity, enabling accurate identification of granulocytic populations in peripheral blood, bone marrow, or cultured cell preparations. This approach is particularly valuable for distinguishing myeloid lineage cells from lymphoid populations during immune profiling experiments and hematopoietic differentiation studies.
Myeloperoxidase Antibody for FACS can be incorporated into multiparameter flow cytometry panels alongside established leukocyte surface markers such as CD45, CD11b, CD15, or other lineage-specific markers. Combining intracellular MPO detection with cell surface immunophenotyping allows detailed characterization of granulocytes, immature myeloid cells, and inflammatory cell subsets within complex biological samples. These multiparameter flow cytometry strategies are widely used to study immune responses, granulocyte development, and changes in myeloid cell composition under inflammatory or disease conditions.
Flow cytometry detection of MPO is also useful in studies examining neutrophil recruitment, immune activation, and tumor-associated inflammation. Quantitative FACS analysis can measure shifts in MPO-positive populations during infection, tissue injury, or immune-mediated disease. Because flow cytometry provides both high sensitivity and rapid single-cell analysis, the Myeloperoxidase Antibody for FACS serves as a powerful tool for identifying and quantifying myeloid lineage cells in immunology, hematology, and inflammatory disease research.
Optimal dilution of the Myeloperoxidase Antibody for FACS should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids K556-I697) was used as the immunogen for the Myeloperoxidase antibody.
After reconstitution, the Myeloperoxidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
MPO antibody, Myeloperoxidase antibody, Myeloid peroxidase antibody, Neutrophil MPO antibody
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