- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Myeloperoxidase Antibody for IHC / MPO Immunohistochemistry Antibody [clone MSVA-692M] (V6098)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V6098-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 599.50 | |
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V6098-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone Name | MSVA-692M |
| UniProt | P05164 |
| Localization | Lysosome |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This MPO/Myeloperoxidase antibody is available for research use only. |
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Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme encoded by the MPO gene and predominantly expressed in neutrophils and other cells of the myeloid lineage. Myeloperoxidase Antibody for IHC / MPO Immunohistochemistry Antibody (clone MSVA-692M) enables visualization of MPO expression in formalin-fixed, paraffin-embedded tissue sections using immunohistochemistry. This approach allows researchers to identify MPO-positive myeloid cells within the histological architecture of normal tissues and tumors.
MPO antibody, also referred to as Myeloperoxidase antibody or myeloid peroxidase antibody in the literature, detects an abundant cytoplasmic enzyme localized within the azurophilic granules of neutrophils and other granulocytic cells. Because of this restricted expression pattern, immunohistochemistry staining with Myeloperoxidase Antibody for IHC highlights granulocytes and related myeloid lineage cells in tissue sections. The staining pattern typically appears as granular cytoplasmic labeling corresponding to MPO-containing granules.
Immunohistochemistry analysis with MPO antibodies is widely used in hematopathology and cancer research to identify cells of myeloid origin. MPO immunohistochemistry staining can distinguish neutrophil lineage cells and is frequently used to evaluate inflammatory infiltrates or characterize myeloid differentiation in tumor or hematologic specimens. Cytoplasmic MPO staining within granulocytes provides a clear histological marker of myeloid cell populations.
Immunohistochemistry also allows investigators to examine MPO-positive cells within the context of tissue architecture. In normal tissues, MPO-positive granulocytes may be observed within bone marrow or circulating within blood-rich organs. In tumor samples or inflammatory tissues, immunohistochemistry staining may highlight infiltrating neutrophils or other MPO-expressing cells present within the tumor microenvironment.
Myeloperoxidase Antibody for IHC (clone MSVA-692M) has been evaluated by immunohistochemistry across a wide range of normal and cancer tissues using tissue microarray analysis. The immunohistochemistry staining patterns observed with this antibody align with Myeloperoxidase / MPO expression data reported in the Human Protein Atlas, supporting its use for histological detection of MPO-expressing cells. This antibody therefore supports immunohistochemistry-based visualization of MPO-positive myeloid cells in tissue sections.
1. Optimal dilution of the Myeloperoxidase Antibody for IHC should be determined by the researcher.
2. This MPO/Myeloperoxidase antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
A recombinant fragment of human MPO protein (exact sequence is proprietary) was used as the immuongen for the MPO/Myeloperoxidase antibody.
MPO/Myeloperoxidase antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
MPO antibody, Myeloperoxidase antibody, Myeloperoxidase MPO antibody, Myeloid peroxidase antibody, Myeloperoxidase enzyme antibody
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