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Home >> Antibodies >> MYDGF Antibody / Myeloid-derived growth factor

MYDGF Antibody / Myeloid-derived growth factor (RQ8382)

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  Catalog No Formulation Size Price (USD)  
Image RQ8382 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Western blot testing of 1) rat liver, 2) rat NRK, 3) mouse ovary, 4) mouse stomach and 5) mouse liver tissue lysate with MYDGF antibody. Predicted molecular weight ~19 kDa, commonly observed at 14-19 kDa.
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Western blot testing of 1) rat liver, 2) rat NRK, 3) mouse ovary, 4) mouse stomach and 5) mouse liver tissue lysate with MYDGF antibody. Predicted molecular weight ~19 kDa, commonly observed at 14-19 kDa.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HepG2, 4) PC-3, 5) U-251, 6) ThP-1, 7) A431 and 8) MCF-7 cell lysate with MYDGF antibody. Predicted molecular weight ~19 kDa, commonly observed at 14-19 kDa.
IHC staining of FFPE human liver cancer tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder urothelial carcinoma tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with MYDGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of fixed and permeabilized human JK cells with MYDGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MYDGF antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q969H8
Localization Cytoplasm, secreted
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This MYDGF antibody is available for research use only.
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Description

Human MYDGF, also called Myeloid-derived growth factor, Stromal cell-derived growth factor SF20 and C19orf10, and formerly known as IL25, is a bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. MYDGF stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis.

Application Notes

Optimal dilution of the MYDGF antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (V32-L173) was used as the immunogen for the MYDGF antibody.

Storage

After reconstitution, the MYDGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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