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Home >> Antibodies >> MXI1 Antibody / Max-interacting protein 1 / BHLHC11

MXI1 Antibody / Max-interacting protein 1 / BHLHC11 [clone PCRP-MXI1-1A3] (V9204)

  Catalog No Formulation Size Price (USD)  
Image V9204-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9204-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9204SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human HeLa cells using MXI1 antibody (green, clone PCRP-MXI1-1A3) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with MXI1 antibody (blue, clone PCRP-MXI1-1A3), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free MXI1 antibody (clone PCRP-MXI1-1A3) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MXI1 antibody (clone PCRP-MXI1-1A3). These results demonstrate the foremost specificity of the PCRP-MXI1-1A3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name PCRP-MXI1-1A3
Purity Protein A/G affinity
UniProt P50539
Localization Nucleus, cytoplasm
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Limitations This MXI1 antibody is available for research use only.
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Description

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (also designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad- binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

Application Notes

Optimal dilution of the MXI1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human MXI1 protein was used as the immunogen for the MXI1 antibody.

Storage

Aliquot the MXI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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