MX1 Antibody / Antiviral Response Protein Antibody (FY13028)

MX1 Antibody Nuclear Localization IF. Immunofluorescent staining of SiHa cells using MX1 Antibody / Antiviral Response Protein Antibody demonstrates strong green fluorescence predominantly localized within the nucleus and perinuclear compartment. Alpha Tubulin is visualized in red, highlighting the cytoskeletal network, while MX1 staining reveals expression of the interferon-inducible antiviral response protein. MX1 (Myxovirus Resistance Protein 1) is a dynamin-like GTPase that functions downstream of interferon signaling pathways and contributes to innate immune defense by restricting viral replication and supporting host antiviral responses. The observed staining pattern is consistent with the intracellular localization of MX1 and supports the utility of this antibody for immunofluorescent detection of antiviral response pathways and interferon-regulated proteins. Enzyme-mediated antigen retrieval was performed for 15 minutes prior to overnight primary antibody incubation and fluorescent secondary antibody detection.

MX1 Antibody Human Mouse Rat WB. Western blot analysis of human whole cell lysate (lane 1), human Caco-2 cells (lane 2), human SiHa cells (lane 3), human PC-3 cells (lane 4), rat PC-12 cells (lane 5), and mouse RAW264.7 cells (lane 6) using MX1 Antibody / Antiviral Response Protein Antibody demonstrates a prominent band at approximately 76 kDa, consistent with the expected molecular weight of MX1. Also known as Myxovirus Resistance Protein 1 (MxA), MX1 is an interferon-inducible dynamin-like GTPase that functions as a key mediator of innate antiviral immunity. MX1 is activated downstream of interferon signaling pathways and contributes to host defense by restricting viral replication and supporting antiviral response mechanisms. Detection of a consistent ~76 kDa band across human, rat, and mouse cell lysates supports the utility of this antibody for cross-species western blot analysis of MX1 expression. Electrophoresis was performed on a 10% SDS-PAGE gel followed by nitrocellulose transfer and HRP-ECL detection.

MX1 Antibody SiHa FACS. Flow cytometric analysis of fixed and permeabilized SiHa cells using MX1 Antibody / Antiviral Response Protein Antibody demonstrates a pronounced rightward shift of the MX1-positive population (blue) relative to both the isotype control (green) and unstained control (red), consistent with intracellular expression of MX1. Also known as Myxovirus Resistance Protein 1 (MxA), MX1 is an interferon-inducible dynamin-like GTPase that functions as a key mediator of innate antiviral immunity and host defense. MX1 is activated downstream of interferon signaling pathways and contributes to restriction of viral replication through multiple antiviral mechanisms. The distinct fluorescence shift supports the utility of this antibody for flow cytometric detection of MX1 expression and evaluation of interferon-responsive antiviral pathways. Cells were fixed, permeabilized, and stained using rabbit anti-MX1 antibody followed by DyLight 488-conjugated secondary antibody detection.