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Home >> Antibodies >> MVP Antibody

MVP Antibody [clone 8B12] (RQ5611)

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  Catalog No Formulation Size Price (USD)  
Image RQ5611 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) U-2 OS, 3) HepG2, 4) ThP-1, 5) HeLa, 6) SW620, 7) rat RH35 and 8) mouse RAW264.7 lysate with MVP antibody. Observed molecular weight: 104~110 kDa.
Flow cytometry testing of human A431 cells with MVP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MVP antibody.
Flow cytometry testing of human A549 cells with MVP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MVP antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 8B12
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q14764
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This MVP antibody is available for research use only.
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Description

Major vault protein is a protein that in humans is encoded by the MVP gene. This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.

Application Notes

Optimal dilution of the MVP antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids A2-H259) was used as the immunogen for the MVP antibody.

Storage

After reconstitution, the MVP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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