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Home >> Antibodies >> MVP Antibody / Major vault protein

MVP Antibody / Major vault protein [clone MSVA-163M] (V6144)

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  Catalog No Formulation Size Price (USD)  
Image V6144-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
Image
V6144-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Major vault protein Mouse Monoclonal Antibody (MSVA-163M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name MSVA-163M
UniProt Q86V7
Localization Cytoplasm, Nuclear pore complex, Nucleus, Perinuclear region
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This MVP/Major vault protein antibody is available for research use only.
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Application Notes

1. Optimal dilution of the MVP/Major vault protein antibody should be determined by the researcher.

2. This MVP/Major vault protein antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragment of human CD163 protein (around amino acids 43-196) (exact sequence is proprietary) was used as the immuongen for the MVP/Major vault protein antibody.

Storage

MVP/Major vault protein antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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