- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MutL Homolog 1 Antibody / MLH1 [clone rMLH1/6285] (V4038)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4038-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 519 | |
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V4038-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 229 | |
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V4038SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rMLH1/6285 |
| Purity | Protein A/G affinity |
| UniProt | P40692 |
| Localization | Nucleus |
| Applications | Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This MutL Homolog 1 antibody is available for research use only. |
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This MAb recognizes a protein of 83kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
Optimal dilution of the MutL Homolog 1 antibody should be determined by the researcher.
Recombinant full-length human MLH1 protein was used as the immunogen for the MutL Homolog 1 antibody.
Aliquot the MutL Homolog 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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