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Home >> Antibodies >> Muscle Actin Antibody (Skeletal/Cardiac/Smooth)

Muscle Actin Antibody (Skeletal/Cardiac/Smooth) [clone rMSA/953] (V8843)

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  Catalog No Formulation Size Price (USD)  
Image V8843-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8843-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8843SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Western blot testing of human skeletal muscle tissue lysate using Muscle Actin antibody (clone rMSA/953). Predicted molecular weight ~45 kDa.
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Western blot testing of human skeletal muscle tissue lysate using Muscle Actin antibody (clone rMSA/953). Predicted molecular weight ~45 kDa.
SDS-PAGE analysis of purified, BSA-free Muscle Actin antibody (clone rMSA/953) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rMSA/953
Purity Protein A/G affinity
UniProt P62736 , P68032 , P68133
Localization Cytoplasm
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-2ug/ml
Limitations This Muscle Actin antibody is available for research use only.
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Description

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Application Notes

Optimal dilution of the Muscle Actin antibody should be determined by the researcher.

Immunogen

Recombinant protein was used as the immunogen for the Muscle Actin antibody.

Storage

Aliquot the Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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