Muscle Actin Antibody HHF35 / Pan Muscle Actin Antibody [clone HHF35] (V2318)

Description

Muscle Actin Antibody HHF35 recognizes muscle-associated actin isoforms expressed in skeletal, cardiac, and smooth muscle cells. Actins are highly conserved cytoskeletal proteins responsible for maintaining cellular structure, generating contractile force, and supporting cell movement. The muscle-specific actin family includes alpha-skeletal actin (ACTA1), alpha-cardiac actin (ACTC1), alpha-smooth muscle actin (ACTA2), and gamma-smooth muscle actin (ACTG2). These proteins contribute to the specialized contractile machinery of different muscle tissues and serve as markers of muscle differentiation.

Actin monomers polymerize into filamentous actin (F-actin), forming structures that interact with myosin to produce mechanical force. In skeletal and cardiac muscle, actin filaments are organized into sarcomeres, where their interactions with myosin drive contraction. Smooth muscle cells contain contractile actin networks that regulate vascular tone, gastrointestinal motility, and other involuntary movements. Although muscle actin isoforms share considerable sequence similarity, their expression patterns and functional specialization differ among tissues.

Clone HHF35 is a widely established monoclonal antibody that recognizes muscle-specific alpha and gamma actin isoforms. Its broad reactivity makes it useful as a Pan Muscle Actin Antibody for identifying muscle differentiation across multiple tissue types. Unlike antibodies directed against a single actin isoform, HHF35 recognizes actins associated with skeletal, cardiac, and smooth muscle lineages. The antibody generally does not react with nonmuscle mesenchymal cells, apart from certain myoepithelial populations.

Muscle actin expression is particularly relevant to the characterization of soft tissue tumors. Leiomyomas and leiomyosarcomas frequently express smooth muscle-associated actins, while rhabdomyomas and rhabdomyosarcomas exhibit skeletal muscle differentiation. Immunohistochemical detection of muscle actin can therefore contribute to the characterization of tumor phenotypes when interpreted alongside other lineage markers, including desmin, myogenin, and smooth muscle myosin. Expression patterns may vary with tumor differentiation and tissue context.

NSJ Bioreagents offers clone HHF35 for research investigating muscle differentiation, cytoskeletal organization, and muscle-associated tumor markers. A Muscle Actin Antibody HHF35 provides broad detection of muscle-specific actin isoforms in studies of normal and neoplastic muscle tissues.

For additional antibodies targeting muscle-specific and nonmuscle actin isoforms involved in cytoskeletal organization, cellular structure, and contraction, explore our Actin Antibodies collection.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle Actin Antibody HHF35 / Pan Muscle Actin Antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

SDS extract of human myocardium was used as the immunogen for this Muscle Actin Antibody HHF35.

Storage

Store the Muscle Actin Antibody HHF35 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Muscle actin antibodyPan Muscle Actin antibody, Muscle Specific Actin antibody, Muscle Actin HHF35 antibody, Muscle Alpha Actin antibody, Muscle Gamma Actin antibody, Sarcomeric Actin antibody, Smooth Muscle Actin antibody, Cardiac Muscle Actin antibody, Skeletal Muscle Actin antibody