MUNC18-1 Antibody / Synaptic Vesicle Exocytosis Protein Antibody (R32158)

MUNC18-1 Antibody Human HeLa Cell IF. Immunofluorescent staining of FFPE human HeLa cells with MUNC18-1 antibody (red). Heat-induced epitope retrieval (HIER) was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei were counterstained with DAPI (blue). MUNC18-1 staining is predominantly cytoplasmic with a punctate distribution throughout the cells, consistent with the expected intracellular localization of STXBP1. The negative control, in which the primary antibody was omitted, showed no specific fluorescence.

MUNC18-1 Antibody Human Glioma IHC. Immunohistochemical staining of FFPE human glioma tissue with MUNC18-1 antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes and allowing them to cool prior to staining. Strong MUNC18-1 immunoreactivity is observed predominantly within the cytoplasm and along the cell membranes of glioma cells, consistent with the expected intracellular localization of STXBP1. Cell nuclei are counterstained with hematoxylin (blue).

MUNC18-1 Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue with MUNC18-1 antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes and allowing them to cool prior to staining. Strong MUNC18-1 immunoreactivity is observed throughout the brain parenchyma, with prominent cytoplasmic staining of neuronal cell bodies and processes, consistent with the expected neuronal expression pattern of STXBP1. Cell nuclei are counterstained with hematoxylin (blue).

MUNC18-1 Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue with MUNC18-1 antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes and allowing them to cool prior to staining. Strong MUNC18-1 immunoreactivity is observed predominantly within the cytoplasm of neurons throughout the brain, with diffuse staining extending into the surrounding neuropil, consistent with the expected neuronal localization of STXBP1. Cell nuclei are counterstained with hematoxylin (blue).

Western blot testing of 1) human HeLa, 2) rat brain, 3) rat C6 and 4) mouse brain lysate with MUNC18-1 antibody. Expected molecular weight ~69 kDa.

MUNC18-1 Antibody Human A549 FACS. Flow cytometric analysis of human A549 cells using MUNC18-1 antibody at 1 ug/10^6 cells following blocking with goat sera. Red: cells alone. Green: isotype control. Blue: MUNC18-1 antibody. The MUNC18-1 antibody produces a clear rightward shift in fluorescence intensity relative to the isotype control, demonstrating specific detection of endogenous MUNC18-1/STXBP1 expression in A549 cells.

Munc18-1 Antibody SH-SY5Y IP-WB. Immunoprecipitation of Munc18-1/STXBP1 from human SH-SY5Y whole cell lysate using Munc18-1 antibody. Lane 1: SH-SY5Y whole cell lysate input (30 ug). Lane 2: Rabbit IgG immunoprecipitation negative control. Lane 3: Munc18-1 antibody immunoprecipitation performed using 2 ug antibody and 500 ug SH-SY5Y whole cell lysate. Following immunoprecipitation, samples were analyzed by Western blot using the same Munc18-1 antibody at 0.5 ug/ml. A specific band is detected at approximately 70 kDa in both the input and Munc18-1 immunoprecipitation lanes, consistent with the predicted molecular weight of approximately 67 kDa. The absence of the target band in the IgG control confirms specific enrichment of Munc18-1/STXBP1. The IgG heavy chain is visible at approximately 50 kDa in the immunoprecipitated samples.