MUC5AC Antibody | Mucin-5AC | 45M1 | V2198 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2198-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2198-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2198SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2198IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC testing of FFPE human stomach stained with MUC5AC antibody (clone 45M1).

IHC testing of FFPE human gastric carcinoma stained with MUC5AC antibody (clone 45M1).

IHC testing of FFPE rat stomach stained with MUC5AC antibody (clone 45M1).

Species Reactivity	Human, Mouse, Rat
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	45M1
Purity	Protein G affinity chromatography
Gene ID	4586
Localization	Cytoplasmic
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This MUC5AC antibody is available for research use only.
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Description

This antibody recognizes the peptide core of gastric mucin M1 (>1,000kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.