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Home >> Antibodies >> MUC5AC Antibody

MUC5AC Antibody [clone 45M1] (V8591)

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  Catalog No Formulation Size Price (USD)  
Image V8591BTN 1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ul 519
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IHC staining of FFPE human gastric carcinoma with biotinylated MUC5AC antibody (clone 45M1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric carcinoma with biotinylated MUC5AC antibody (clone 45M1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Biotin Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 45M1
Purity Protein G affinity chromatography
UniProt P98088
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 minutes at RT
Limitations This biotinylated MUC5AC antibody is available for research use only.
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Description

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Application Notes

Optimal dilution of the biotinylated MUC5AC antibody should be determined by the researcher.

Immunogen

An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for the biotinylated MUC5AC antibody.

Storage

Store the biotinylated MUC5AC antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

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