CD146 Antibody OJ79c | Mel-CAM | V2189 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2189-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V2189-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V2189SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

CD146 Antibody OJ79c by SDS-PAGE Under Reducing and Non-Reducing Conditions. SDS-PAGE analysis of purified, BSA-free mouse monoclonal CD146 antibody (OJ79c) under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. The non-reduced antibody migrates predominantly at approximately 150 kDa, consistent with intact IgG, while reduction produces heavy and light chains at approximately 50 kDa and 25 kDa, respectively. The results demonstrate the structural integrity and purity of clone OJ79c, which recognizes the melanoma cell adhesion molecule CD146, also known as Mel-CAM and MUC18.

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	OJ79c
Purity	Protein G purified antibody
Buffer	1X PBS, pH 7.4
Gene ID	4162
Localization	Cell surface, cytoplasm
Applications	Flow Cytometry : 0.5-1ug/10^6 cells Immunofluorescence : 0.5-1ug/ml
Limitations	This CD146 Antibody OJ79c / Mel-CAM Antibody is available for research use only.
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Description

CD146 Antibody OJ79c recognizes CD146, a cell surface glycoprotein belonging to the immunoglobulin superfamily. Also known as melanoma cell adhesion molecule (MCAM), MUC18, and Mel-CAM, this protein is encoded by the MCAM gene on human chromosome 11. Originally identified as an antigen expressed by melanoma cells, CD146 is now recognized as an important adhesion molecule involved in vascular biology, cellular migration, and interactions between neighboring cells. Its expression in normal and malignant tissues makes it relevant to investigations of endothelial function, tumor progression, and cellular differentiation.

CD146 is a type I transmembrane protein containing five extracellular immunoglobulin-like domains, a single membrane-spanning region, and a cytoplasmic tail involved in intracellular signaling. Extensive glycosylation contributes to its relatively high apparent molecular weight, while alternative splicing generates isoforms with different cytoplasmic domains. These structural features influence interactions with cellular binding partners and signaling pathways involved in adhesion, cytoskeletal organization, and migration.

Mel-CAM is expressed by vascular endothelial cells, smooth muscle cells, pericytes, and subsets of mesenchymal stromal cells. In vascular tissues, CD146 contributes to endothelial organization, cell-cell interactions, and processes associated with angiogenesis. It is also expressed by certain activated immune cell populations, including subsets of T lymphocytes, where it has been investigated as a marker associated with cellular activation and migration.

In melanoma research, CD146 expression has been associated with invasive behavior, increased cellular motility, and metastatic progression. Experimental studies suggest that MCAM participates in signaling pathways that influence interactions with the extracellular matrix and surrounding stromal cells. Expression of MUC18 has also been reported in other malignancies, although its biological significance varies according to tumor type and cellular context.

Because CD146 is present in both normal vascular tissues and certain tumors, its detection can help characterize cell surface protein expression and investigate changes associated with cellular differentiation. Flow cytometry provides information about CD146 expression in cell populations, while immunofluorescence can reveal its distribution within individual cells. These approaches are useful for studying the relationship between MCAM expression, cellular phenotype, and adhesion-associated signaling.

A CD146 Antibody OJ79c from NSJ Bioreagents supports research investigating Mel-CAM expression, endothelial cell biology, melanoma-associated adhesion mechanisms, and the regulation of cellular migration.

For an alternative antibody targeting the same cell adhesion molecule, see our MCAM Antibody MCAM/3046, a mouse monoclonal antibody featuring human melanoma IHC and protein microarray specificity validation.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD146 Antibody OJ79c / Mel-CAM Antibody to be titered up or down for optimal performance.

Immunogen

Recombinant human protein was used as the immunogen for this CD146 Antibody OJ79c.

Storage

Store the CD146 Antibody OJ79c at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, MCAM, Melanoma Adhesion Molecule, Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1, CD146 antibody

CD146 Antibody OJ79c / Mel-CAM Antibody [clone OJ79c] (V2189)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents