MUC1 Antibody / Mouse Monoclonal [clone 115D8] (V3962)

Description

This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human milk fat globule membranes were used as the immunogen for this MUC1 antibody.

Storage

Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).