MUC-1 Antibody | Mucin 1 | Clone MUC1/955

	Catalog No	Formulation	Size	Price (USD)
	V2372-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	539
Formulation
	V2372-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	249
Formulation
	V2372SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	539
Formulation
	V2372IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	539
Formulation

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IHC staining of FFPE human endometrial carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human lung carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with MUC-1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human T47D cell lysate with MUC-1 antibody (clone MUC1/955). Expected molecular weight: 120-500 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free MUC-1 antibody (clone MUC1/955) as confirmation of integrity and purity.

Western blot testing of human MCF7 cell lysate with MUC-1 antibody (clone MUC1/955). Expected molecular weight: 120-500 kDa depending on glycosylation level.

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	MUC1/955
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	4582
RRID	AB_2864392
Localization	Cytoplasmic and cell surface
Applications	Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1) Immunofluorescence : see citations 1 & 2
Limitations	This MUC-1 antibody is available for research use only.
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Description

MUC-1 antibody clone MUC1/955 is a monoclonal antibody that targets mucin-1, a transmembrane glycoprotein expressed on the apical surface of epithelial cells. MUC-1 is heavily glycosylated and plays roles in protecting epithelial barriers, mediating cell adhesion, and modulating signaling pathways. In healthy tissues, MUC-1 expression is polarized, while in cancers it becomes overexpressed and loses apical restriction, contributing to tumor invasion and metastasis. NSJ Bioreagents provides MUC-1 antibody clone MUC1/955 as a trusted reagent for studies in cancer biology, immunology, and epithelial physiology.

MUC-1 antibody clone MUC1/955 produces strong membranous and cytoplasmic staining in glandular epithelial tissues, such as breast, pancreas, and gastrointestinal tract. In pathology, clone MUC1/955 is commonly used as a marker for adenocarcinomas, including breast, ovarian, and pancreatic cancers. Its detection provides important diagnostic information, as aberrant MUC-1 expression correlates with tumor progression, poor prognosis, and metastatic potential.

In cancer research, MUC-1 antibody clone MUC1/955 has been employed to study how tumor-associated MUC-1 differs structurally and functionally from normal epithelial MUC-1. The altered glycosylation of tumor MUC-1 creates novel epitopes, making it a target for immunotherapy. Studies using clone MUC1/955 have contributed to vaccine development, monoclonal antibody therapies, and CAR T cell approaches aimed at targeting tumor-associated MUC-1.

Beyond oncology, MUC-1 antibody clone MUC1/955 supports investigations into epithelial defense mechanisms. MUC-1 modulates interactions with pathogens, including viruses and bacteria, and its detection has been used to clarify how mucins contribute to innate immunity at mucosal surfaces.

In reproductive biology, MUC-1 antibody clone MUC1/955 has been applied to uterine and embryonic studies, as MUC-1 expression at the endometrium regulates implantation. Altered MUC-1 dynamics in fertility disorders highlight its broader role in human physiology.

This antibody is validated for tissue and cell-based studies, delivering reproducible results in both normal and diseased tissues. Alternate names include epithelial membrane antigen antibody, episialin antibody, and carcinoma-associated mucin antibody.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC-1 antibody to be titered up or down for optimal performance.

>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.