mtTFA Antibody / TFAM / Mitochondrial transcription factor A (R32176)

Description

Mitochondrial transcription factor A, commonly referred to as mtTFA or TFAM, is a key nuclear-encoded protein that plays an essential role in the maintenance and expression of mitochondrial DNA. It is a member of the high mobility group (HMG) protein family and functions by binding to mitochondrial DNA to initiate transcription and aid in replication. mtTFA is also involved in the compaction and structural organization of mitochondrial nucleoids.

The mtTFA protein is critical for mitochondrial biogenesis and energy production, making it an important target in studies related to metabolism, aging, neurodegenerative diseases, and cancer. Research indicates that mtTFA levels often correlate with mitochondrial content and function in cells, making it a widely used marker in mitochondrial biology. Multiple isoforms of mtTFA have been identified, which may have distinct regulatory roles or tissue-specific expression patterns, underscoring the importance of precise detection methods.

A well-validated mtTFA antibody is indispensable for mitochondrial research. The mtTFA antibody can be employed in western blot assays to quantify expression levels, in immunofluorescence to visualize mitochondrial localization, and in immunohistochemistry to assess tissue distribution. NSJ Bioreagents provides a high-specificity mtTFA antibody that delivers consistent and reliable results across a variety of applications in both basic and translational research.

Application Notes

Optimal dilution of the mtTFA antibody should be determined by the researcher.

Immunogen

Amino acids EMKSWEEQMIEVGRKDLLRRTIKKQRKY of human mtTFA were used as the immunogen for the mtTFA antibody.

Storage

After reconstitution, the mtTFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.