MTCO1 Rodent Antibody | Cytochrome C Oxidase

	Catalog No	Formulation	Size	Price (USD)
	R30377	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

MTCO1 Rodent Antibody by WB in Rat and Mouse Heart Tissue. Western blot analysis of rat heart (lane 1) and mouse heart (lane 2) tissue lysates using rabbit polyclonal MTCO1 antibody. Strong immunoreactive bands are detected at approximately 40 kDa in both samples, below the calculated molecular weight of approximately 57 kDa for cytochrome c oxidase subunit 1. This faster electrophoretic migration has been reported for MTCO1 and is associated with the unusual SDS-PAGE behavior of highly hydrophobic mitochondrial membrane proteins. Incomplete protein unfolding and altered SDS binding may contribute to the lower apparent molecular weight.

MTCO1 Rodent Antibody by IHC in Rat Brain Tissue. Immunohistochemistry staining of FFPE rat brain tissue using rabbit polyclonal MTCO1 antibody. Brown immunoreactivity is observed predominantly in the cytoplasm of neuronal cell bodies and surrounding neuropil, consistent with the mitochondrial localization of cytochrome c oxidase subunit 1. Cell nuclei exhibit comparatively little staining. MTCO1 is an integral component of mitochondrial respiratory complex IV, which participates in electron transport and oxidative phosphorylation.

MTCO1 Rodent Antibody by IHC in Mouse Liver Tissue. Immunohistochemistry staining of FFPE mouse liver tissue using rabbit polyclonal MTCO1 antibody. Brown immunoreactivity is distributed throughout the hepatocyte cytoplasm, displaying a granular staining pattern consistent with the intracellular distribution of mitochondria. Cell nuclei are largely unstained. The widespread cytoplasmic signal reflects detection of cytochrome c oxidase subunit 1, a mitochondrially encoded component of respiratory complex IV involved in oxidative phosphorylation.

MTCO1 Rodent Antibody by IHC in Mouse Spleen Tissue. Immunohistochemistry staining of FFPE mouse spleen tissue using rabbit polyclonal MTCO1 antibody. Brown cytoplasmic immunoreactivity is observed in scattered splenic cells, with variable staining intensity across the tissue. The staining pattern is consistent with the intracellular localization of cytochrome c oxidase subunit 1, an integral mitochondrial membrane protein involved in respiratory chain electron transfer. Cell nuclei exhibit comparatively weak staining.

MTCO1 Rodent Antibody by ICC in Mouse HEPA1-6 Cells. Immunocytochemistry staining of mouse HEPA1-6 hepatoma cells using rabbit polyclonal MTCO1 antibody. Strong brown immunoreactivity is observed throughout the cytoplasm, with a granular distribution and comparatively little nuclear staining. This pattern is consistent with the mitochondrial localization of cytochrome c oxidase subunit 1, a catalytic component of respiratory complex IV responsible for the terminal electron transfer to molecular oxygen.

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MTCO1 Rodent Antibody by WB in Rat and Mouse Heart Tissue. Western blot analysis of rat heart (lane 1) and mouse heart (lane 2) tissue lysates using rabbit polyclonal MTCO1 antibody. Strong immunoreactive bands are detected at approximately 40 kDa in both samples, below the calculated molecular weight of approximately 57 kDa for cytochrome c oxidase subunit 1. This faster electrophoretic migration has been reported for MTCO1 and is associated with the unusual SDS-PAGE behavior of highly hydrophobic mitochondrial membrane proteins. Incomplete protein unfolding and altered SDS binding may contribute to the lower apparent molecular weight.

MTCO1 Rodent Antibody by IHC in Rat Brain Tissue. Immunohistochemistry staining of FFPE rat brain tissue using rabbit polyclonal MTCO1 antibody. Brown immunoreactivity is observed predominantly in the cytoplasm of neuronal cell bodies and surrounding neuropil, consistent with the mitochondrial localization of cytochrome c oxidase subunit 1. Cell nuclei exhibit comparatively little staining. MTCO1 is an integral component of mitochondrial respiratory complex IV, which participates in electron transport and oxidative phosphorylation.

MTCO1 Rodent Antibody by IHC in Mouse Liver Tissue. Immunohistochemistry staining of FFPE mouse liver tissue using rabbit polyclonal MTCO1 antibody. Brown immunoreactivity is distributed throughout the hepatocyte cytoplasm, displaying a granular staining pattern consistent with the intracellular distribution of mitochondria. Cell nuclei are largely unstained. The widespread cytoplasmic signal reflects detection of cytochrome c oxidase subunit 1, a mitochondrially encoded component of respiratory complex IV involved in oxidative phosphorylation.

MTCO1 Rodent Antibody by IHC in Mouse Spleen Tissue. Immunohistochemistry staining of FFPE mouse spleen tissue using rabbit polyclonal MTCO1 antibody. Brown cytoplasmic immunoreactivity is observed in scattered splenic cells, with variable staining intensity across the tissue. The staining pattern is consistent with the intracellular localization of cytochrome c oxidase subunit 1, an integral mitochondrial membrane protein involved in respiratory chain electron transfer. Cell nuclei exhibit comparatively weak staining.

MTCO1 Rodent Antibody by ICC in Mouse HEPA1-6 Cells. Immunocytochemistry staining of mouse HEPA1-6 hepatoma cells using rabbit polyclonal MTCO1 antibody. Strong brown immunoreactivity is observed throughout the cytoplasm, with a granular distribution and comparatively little nuclear staining. This pattern is consistent with the mitochondrial localization of cytochrome c oxidase subunit 1, a catalytic component of respiratory complex IV responsible for the terminal electron transfer to molecular oxygen.

Availability	1-3 business days
Species Reactivity	Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt	P00397
Localization	Cytoplasmic (mitochondrial)
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml Immunocytochemistry : 0.5-1ug/ml
Limitations	This MTCO1 Rodent Antibody / Cytochrome C Oxidase Subunit 1 Antibody is available for research use only.
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Description

MTCO1 Rodent Antibody recognizes mitochondrially encoded cytochrome c oxidase subunit I, an integral membrane protein that forms part of mitochondrial respiratory complex IV. Also known as MT-CO1, COX1, and cytochrome c oxidase subunit 1, this protein is encoded by mitochondrial DNA rather than the nuclear genome. MTCO1 is a highly conserved component of the mitochondrial electron transport chain and plays a central role in oxidative phosphorylation, the process responsible for generating most cellular ATP under aerobic conditions.

Cytochrome c oxidase, also called complex IV, is the terminal enzyme of the mitochondrial respiratory chain. It receives electrons from reduced cytochrome c and transfers them to molecular oxygen, producing water. This electron transfer is coupled to proton translocation across the inner mitochondrial membrane, contributing to the electrochemical gradient used by ATP synthase to produce ATP. Efficient complex IV activity is therefore essential for maintaining mitochondrial energy metabolism.

MTCO1 forms a major catalytic component of cytochrome c oxidase and contains the heme a and heme a3-CuB centers involved in electron transfer and oxygen reduction. The protein spans the inner mitochondrial membrane through multiple transmembrane helices and associates with other mitochondrially encoded and nuclear-encoded subunits to form the functional enzyme complex. Proper assembly of these components is necessary for respiratory chain stability and activity.

Because MTCO1 is encoded by mitochondrial DNA, its synthesis depends on mitochondrial transcription and translation machinery. Mutations affecting the MT-CO1 gene can disrupt complex IV activity and impair oxidative phosphorylation. Deficiencies in mitochondrial respiratory function are associated with a range of metabolic and neuromuscular disorders, particularly in tissues with high energy requirements, including skeletal muscle, cardiac muscle, and the nervous system.

MTCO1 expression is commonly investigated in studies of mitochondrial abundance, respiratory chain organization, and oxidative phosphorylation. However, protein abundance alone does not establish complex IV enzymatic activity or overall mitochondrial function. Changes in MTCO1 levels may reflect differences in mitochondrial content, protein synthesis, or respiratory complex stability, depending on the experimental system.

An MTCO1 Rodent Antibody from NSJ Bioreagents supports investigations of mitochondrial respiratory chain proteins, complex IV expression, and oxidative phosphorylation in rodent research models.

For an alternative antibody targeting the same mitochondrial respiratory chain protein, see our MTCO1 Antibody page.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the MTCO1 Rodent Antibody / Cytochrome C Oxidase Subunit 1 Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of mouse MTCO1 (PYHTFEEPTYVKVK) was used as the immunogen for this MTCO1 Rodent Antibody.

Storage

After reconstitution, the MTCO1 Rodent Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

MT-CO1 antibody, Cytochrome C Oxidase Subunit I antibody, Cytochrome C Oxidase Subunit 1 antibody, COX1 antibody, COI antibody, Mitochondrially Encoded Cytochrome C Oxidase I antibody

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MTCO1 Rodent Antibody / Cytochrome C Oxidase Subunit 1 Antibody (R30377)

Description

Application Notes

Immunogen

Storage

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NSJ Bioreagents