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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MTCO1 Antibody / Cytochrome C Oxidase Subunit I Antibody (R30376)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R30376 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal |
| UniProt | P00395 |
| Localization | Cytoplasmic (mitochondrial) |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml Immunocytochemistry : 0.5-1ug/ml |
| Limitations | This MTCO1 Antibody / Cytochrome C Oxidase Subunit I Antibody is available for research use only. |
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MTCO1 Antibody recognizes mitochondrially encoded cytochrome c oxidase subunit I, a highly conserved protein that forms the catalytic core of mitochondrial respiratory complex IV. Also known as MT-CO1, COX1, and cytochrome c oxidase subunit 1, this protein is encoded by the mitochondrial MT-CO1 gene and is located within the inner mitochondrial membrane. MTCO1 is an essential component of the electron transport chain, where it contributes to the transfer of electrons to molecular oxygen during oxidative phosphorylation.
Cytochrome c oxidase, also called complex IV, is the terminal enzyme of the mitochondrial respiratory chain. It accepts electrons from reduced cytochrome c and transfers them to oxygen, producing water. This process is coupled to proton translocation across the inner mitochondrial membrane, helping establish the electrochemical gradient required for ATP synthesis. Because oxidative phosphorylation provides much of the ATP needed for cellular activities, efficient complex IV function is particularly important in metabolically active tissues, including the heart, skeletal muscle, brain, and liver.
Cytochrome c oxidase subunit I contains multiple transmembrane helices and houses the heme a and heme a3-CuB catalytic centers responsible for electron transfer and oxygen reduction. The protein associates with other mitochondrially encoded and nuclear-encoded subunits to form the mature respiratory complex. Proper synthesis, membrane insertion, and assembly of MTCO1 are necessary for complex IV stability and mitochondrial respiratory activity.
Unlike most mitochondrial proteins, MTCO1 is synthesized within mitochondria using the mitochondrial genetic code. Its expression depends on mitochondrial DNA integrity, transcription, and translation. Pathogenic variants affecting MT-CO1 can impair respiratory chain function and contribute to mitochondrial disorders characterized by deficient cellular energy production. The consequences of these defects vary according to mutation severity, tissue energy requirements, and the proportion of affected mitochondrial genomes.
MTCO1 is frequently investigated as a marker of mitochondrial respiratory complex IV expression. Western blotting, immunohistochemistry, and immunocytochemistry can provide information about its abundance and distribution in different experimental systems. However, MTCO1 protein levels do not independently establish complex IV enzymatic activity, and functional studies may be required to evaluate mitochondrial respiratory performance.
An MTCO1 Antibody from NSJ Bioreagents supports research investigating mitochondrial respiratory chain organization, cytochrome c oxidase expression, oxidative phosphorylation, and the molecular mechanisms underlying mitochondrial dysfunction.
For additional antibodies targeting mitochondrial respiratory chain proteins and markers of mitochondrial function, explore our Mitochondria Marker Antibody collection.
The stated application concentrations are suggested starting amounts. Titration of the MTCO1 Antibody / Cytochrome C Oxidase Subunit I Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of the human protein (PYHTFEEPVYMKS) was used as the immunogen for this MTCO1 antibody.
After reconstitution, the MTCO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
MT-CO1 antibody, Cytochrome C Oxidase Subunit I antibody, Cytochrome C Oxidase Subunit 1 antibody, COX1 antibody, COI antibody, Mitochondrially Encoded Cytochrome C Oxidase I antibody, Complex IV Subunit 1 antibody
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