MTAP Polyclonal Antibody / Methylthioadenosine Phosphorylase Antibody (FY13688)

MTAP Polyclonal Antibody IF Staining of Human PC-3 Cells. Immunofluorescence analysis of human PC-3 cells using Methylthioadenosine Phosphorylase Antibody at 5 ug/ml. Red fluorescence indicates MTAP immunoreactivity distributed throughout the cytoplasm, with staining extending into perinuclear regions. DAPI counterstaining identifies nuclei in blue. Enzyme-mediated antigen retrieval was performed for 15 minutes, followed by overnight primary antibody incubation at 4oC and detection with a DyLight 550-conjugated secondary antibody.

MTAP Polyclonal Antibody Western Blot Analysis of HeLa, 293T, Raji, PC-12 and RAW264.7 Cell Lysates. Western blot analysis using Methylthioadenosine Phosphorylase Antibody at 0.5 ug/ml. Lane 1: human HeLa cells; lane 2: human 293T cells; lane 3: human Raji cells; lane 4: rat PC-12 cells; lane 5: mouse RAW264.7 cells. Each lane contains 30 ug of total protein under reducing conditions. Immunoreactive bands are detected at approximately 31 kDa across all five samples, consistent with the calculated molecular weight of MTAP at approximately 31.2 kDa.

MTAP Polyclonal Antibody Western Blot Validation in HepG2 Wild-Type and MTAP Knockout Cells. Western blot analysis of human HepG2 wild-type (WT, lane 1) and MTAP knockout (KO, lane 2) whole-cell lysates using Methylthioadenosine Phosphorylase Antibody at 0.5 ug/ml. Each lane contains 30 ug of total protein under reducing conditions. An immunoreactive band near 31 kDa is detected in the WT lysate and substantially reduced in the KO sample, supporting antibody specificity for MTAP. The observed molecular weight is consistent with the calculated size of approximately 31.2 kDa. Alpha-Tubulin serves as the loading control.

MTAP Polyclonal Antibody Flow Cytometry Analysis of Human U-2 OS Cells. Flow cytometric analysis of fixed and permeabilized human U-2 OS cells using Methylthioadenosine Phosphorylase Antibody at 1 ug per million cells. The blue histogram represents MTAP antibody-stained cells, the green histogram represents the rabbit IgG isotype control, and the red histogram represents the blank control. The MTAP-stained population displays a rightward fluorescence shift relative to the controls, demonstrating intracellular MTAP immunoreactivity. Cells were fixed with 4% paraformaldehyde, permeabilized, and stained using a DyLight 488-conjugated secondary antibody.