MSLN Antibody / Microarray Specificity Validated Antibody [clone MSLN/3387] (V8693)

MSLN Antibody Mesothelioma IHC. Immunohistochemistry analysis of mesothelin (MSLN) expression in FFPE human mesothelioma. Tumor cells demonstrate membranous HRP-DAB brown staining with focal cytoplasmic signal, consistent with mesothelin cell surface localization and processing. The staining pattern highlights mesothelial tumor cell populations with low background in surrounding stromal elements. The clean, selective signal aligns with microarray specificity validated performance, supporting high-confidence detection of MSLN in tissue sections. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

IHC staining of FFPE human mesothelioma with MSLN antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MSLN Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using clone MSLN/3387 demonstrates highly selective binding to MSLN. The antibody shows a dominant signal for MSLN with a strong Z-score and clear separation from all other proteins, indicating exceptional specificity and minimal cross-reactivity across the proteome.
The Z-score reflects signal intensity relative to the overall array, expressed as standard deviations above the mean. The S-score represents the difference between adjacent ranked Z-scores and provides a measure of target specificity. The large separation between MSLN and secondary targets supports high-confidence detection of mesothelin in complex biological samples.

Western blot testing of human OVCAR-3 cell lysate with specificity validated MSLN antibody. Predicted molecular weight ~70 kDa (precursor), ~40 kDa (processed form).