- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MSH6 Antibody Mouse Monoclonal MTS6-1 [clone MTS6-1] (V7430)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7430-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V7430-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V7430SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 | |
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V7430IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | MTS6-1 |
| Purity | Protein G affinity chromatography |
| UniProt | P52701 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/10^6 cells Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml Prediluted IHC Only Format : incubate for 30 min at RT (1) |
| Limitations | This MSH6 antibody is available for research use only. |
|
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MutS homolog 6 (MSH6), encoded by the MSH6 gene, is a nuclear DNA mismatch repair protein that plays a critical role in maintaining genomic stability during DNA replication. MSH6 Antibody Mouse Monoclonal MTS6-1 recognizes this essential DNA repair factor, which is widely referred to in the literature as MutS homolog 6, GTBP, or G/T mismatch-binding protein. MSH6 functions as part of the MutSalpha heterodimer formed with MutS homolog 2 (MSH2), a complex responsible for detecting base-base mismatches and small insertion-deletion loops that arise during DNA replication. By identifying these replication errors, the MutSalpha complex initiates the DNA mismatch repair pathway, helping preserve genome integrity in proliferating cells.
Within the mismatch repair system, MSH6 serves as the primary mismatch recognition component of the MutSalpha complex. After binding to mismatched nucleotides, the complex recruits downstream repair proteins including MLH1 and PMS2, which coordinate removal of the incorrect DNA strand followed by synthesis of the corrected sequence. This repair mechanism is highly conserved across eukaryotes and is essential for preventing the accumulation of mutations during cell division.
The MSH6 gene is located on chromosome 2p16 and encodes a member of the MutS family of DNA repair proteins. The protein is localized predominantly within the cell nucleus where DNA replication and repair processes occur. Detection of MSH6 expression in tissue-based studies typically reveals nuclear staining in proliferating epithelial cells and lymphoid populations that undergo active DNA replication. This nuclear localization reflects the functional role of MSH6 in recognizing mismatched nucleotides and initiating DNA repair processes.
Alterations in mismatch repair genes can lead to genomic instability and increased susceptibility to tumor development. Loss of MSH6 expression contributes to mismatch repair deficiency and microsatellite instability, molecular features frequently investigated in colorectal carcinoma, endometrial carcinoma, and other malignancies. Mutations in the MSH6 gene have also been associated with hereditary cancer syndromes including Lynch syndrome. Consequently, detection of MSH6 protein expression is widely used in research examining DNA repair pathways, genomic instability, and tumor biology.
Several well-established literature synonyms exist for this mismatch repair protein, including MutS homolog 6, GTBP, and G/T mismatch-binding protein. These alternate names reflect the protein's role in recognizing G/T mismatches generated during DNA replication. A mouse monoclonal antibody such as clone MTS6-1 enables reliable detection of nuclear MSH6 expression in research applications investigating DNA mismatch repair mechanisms and genomic stability.
The stated application concentrations are suggested starting points. Titration of the MSH6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
A portion of amino acids 374-540 from the human protein were used as the immunogen for this MSH6 antibody.
Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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