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Home >> Antibodies >> MSH6 Antibody

MSH6 Antibody [clone MST6-2] (V7592)

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  Catalog No Formulation Size Price (USD)  
Image V7592-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7592-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7592SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7592IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MST6-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MST6-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MST6-2) and Phalloidin (red).
Western blot testing of human HCT116 lysate with MSH6 antibody (clone MST6-2). Expected molecular weight: 120-160 kDa depending on phosphorylation level.
Flow cytometry testing of fixed and permeabilized human MCF-7 cells with MSH6 antibody (clone MST6-2); Red=isotype control, Blue= Estrogen Receptor alpha antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name MST6-2
Purity Protein G affinity chromatography
UniProt P52701
Localization Nucleus
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This MSH6 antibody is available for research use only.
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Description

MSH6 is a component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. [UniProt]

Application Notes

Optimal dilution of the MSH6 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.

Storage

Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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