MSH6 Antibody for IF / MutS homolog 6 immunofluorescence antibody [clone MSH6/3091] (V8011)

MSH6 Antibody for IF staining of PFA-fixed human MCF-7 cells. Immunofluorescence analysis shows strong nuclear green signal corresponding to MutS homolog 6 (MSH6), consistent with the nuclear localization of this DNA mismatch repair protein. Cells were stained with the mouse monoclonal MSH6 antibody clone MSH6/3091 (green), while Phalloidin (red) highlights filamentous actin outlining the cytoskeleton and cell boundaries.

IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MSH6/3091). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human HCT116 lysate with MSH6 antibody (clone MSH6/3091). Expected molecular weight: 120-160 kDa depending on phosphorylation level.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MSH6 antibody (clone MSH6/3091). These results demonstrate the foremost specificity of the MSH6/3091 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free MSH6 antibody (clone MSH6/3091) as confirmation of integrity and purity.