MSH6 Antibody [clone MSH6/3086] (V7566)

Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MSH6/3086) and Phalloidin (red).

IHC staining of FFPE human small intestine with MSH6 antibody (clone MSH6/3086). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MSH6/3086). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human HCT116 lysate with MSH6 antibody (clone MSH6/3086). Expected molecular weight: 120-160 kDa depending on phosphorylation level.

Flow cytometry testing of fixed and permeabilized human MCF-7 cells with MSH6 antibody (clone MSH6/3086); Red=isotype control, Blue= MSH6 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MSH6 antibody (clone MSH6/3086). These results demonstrate the foremost specificity of the MSH6/3086 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free MSH6 antibody (clone MSH6/3086) as confirmation of integrity and purity.