MSH2 Antibody Mouse Monoclonal 6B4F7 / MutS homolog 2 Antibody [clone 6B4F7] (RQ7009)

MSH2 Antibody Mouse Monoclonal 6B4F7. Immunohistochemistry of FFPE human laryngeal squamous cell carcinoma tissue demonstrates strong nuclear staining in tumor epithelial cells, consistent with the nuclear localization of MutS homolog 2 (MSH2), a key DNA mismatch repair protein. Brown chromogenic signal highlights MSH2-positive nuclei across the carcinoma cell population, while surrounding stromal cells show weaker or variable staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH8 EDTA buffer for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human serous adenocarcinoma of the ovary tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

MSH2 Antibody Mouse Monoclonal 6B4F7. Immunofluorescence analysis of FFPE human Caco-2 cells shows strong nuclear staining corresponding to MutS homolog 2 (MSH2) protein localization. Red fluorescent signal highlights MSH2-positive nuclei throughout the cell population, while DAPI counterstain (blue) marks total nuclear DNA for reference. The staining pattern demonstrates clear nuclear accumulation consistent with the role of MSH2 as a DNA mismatch repair protein functioning within the nucleus. Heat-induced epitope retrieval was performed by steaming sections in pH6 citrate buffer for 20 minutes prior to antibody incubation.

Western blot testing of human 1) HeLa, 2) MCF7, 3) A459 and 4) COLO-320 cell lysate with MSH2 antibody. Expected molecular weight ~105 kDa.