MRP3 Antibody Protein Microarray Validated / ABCC3 [clone ABCC3/2971] (V8183)

Immunohistochemistry analysis of MRP3 / ABCC3 antibody (clone ABCC3/2971) in human pancreatic carcinoma tissue. Formalin-fixed, paraffin-embedded tumor demonstrates strong membranous HRP-DAB brown staining in epithelial tumor cells, outlining cell borders consistent with plasma membrane localization of Multidrug resistance-associated protein 3. Stromal elements show minimal staining. Hematoxylin counterstain highlights tumor architecture and nuclei. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

IHC staining of FFPE human pancreatic carcinoma with MRP3 antibody (clone ABCC3/2971). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreatic carcinoma with MRP3 antibody (clone ABCC3/2971). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MRP3 antibody (clone ABCC3/2971). These results demonstrate the foremost specificity of the ABCC3/2971 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.