MRE11 Antibody / DNA Double-Strand Break Repair Protein Antibody (RQ4229)

MRE11 Antibody Human Osteosarcoma Cell IF. Immunofluorescent staining of U2OS cells using MRE11 antibody (red) and alpha tubulin antibody (green) demonstrated predominant nuclear localization of MRE11, consistent with its role as a core component of the MRN complex involved in DNA double-strand break recognition and repair. Cells were treated with enzyme antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with both primary antibodies overnight at 4°C. Cy3-conjugated goat anti-rabbit IgG and Fluoro488-conjugated goat anti-mouse IgG were used as secondary antibodies. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, MRN complex biology, and cancer research.

MRE11 Antibody Human Intestinal Cancer IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using MRE11 antibody demonstrated strong nuclear fluorescence (red), consistent with the localization of MRE11 as a key component of the MRN complex responsible for DNA double-strand break recognition and repair. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by Fluoro550-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI (blue). This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, MRN complex biology, and colorectal cancer research.

MRE11 Antibody Rat Brain IF. Immunofluorescent staining of FFPE rat brain tissue using MRE11 antibody demonstrated prominent nuclear fluorescence (red) in neuronal and glial cells, consistent with the localization of MRE11 as a core component of the MRN complex involved in DNA double-strand break recognition and repair. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by Fluoro550-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI (blue). This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, neurobiology, and MRN complex function.

MRE11 Antibody Human, Rat and Mouse WB. Western blot testing of human HeLa (lane 1), human K562 (lane 2), rat C6 (lane 3), and mouse L929 (lane 4) lysates using MRE11 antibody detected a specific band at approximately 81 kDa, consistent with the expected molecular weight of MRE11. Detection across human and rodent cell lines demonstrates broad species reactivity for this highly conserved DNA repair protein. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, MRN complex biology, and cancer research.

MRE11 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue using MRE11 antibody demonstrated predominantly nuclear staining in tumor cells, consistent with the role of MRE11 as a key component of the MRN complex involved in DNA double-strand break recognition and repair. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, MRN complex biology, and cancer research.

MRE11 Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using MRE11 antibody demonstrated predominantly nuclear staining in malignant epithelial cells, consistent with the localization of MRE11 as a key component of the MRN complex responsible for DNA double-strand break detection and repair. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, MRN complex biology, and colorectal cancer research.

MRE11 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using MRE11 antibody demonstrated predominantly nuclear staining in malignant cells, consistent with the role of MRE11 in DNA double-strand break recognition, DNA end processing, and genome maintenance as a core component of the MRN complex. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating DNA damage response signaling, genome stability, MRN complex biology, and lung cancer research.

MRE11 Antibody Human Oral Squamous Cell Carcinoma IHC. Immunohistochemical staining of FFPE human oral keratinizing squamous cell carcinoma tissue using MRE11 antibody demonstrated predominantly nuclear staining in malignant epithelial cells, consistent with the localization of MRE11 as a key DNA repair nuclease within the MRN complex. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating DNA damage response signaling, genome stability, MRN complex biology, and head and neck cancer research.

MRE11 Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using MRE11 antibody demonstrated predominantly nuclear staining in neuronal and glial cells, consistent with the role of MRE11 as a core component of the MRN complex responsible for DNA double-strand break detection and repair. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This MRE11 Antibody, also called a DNA Double-Strand Break Repair Protein Antibody, is useful for investigating genome stability, DNA damage response signaling, neurobiology, and MRN complex function.