- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MPO Antibody for FACS / Myeloperoxidase Flow Cytometry Antibody (R31487)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R31487 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| Gene ID | 4353 |
| Localization | Nuclear and cytoplasmic |
| Applications | Western Blot : 0.5-1ug/ml Flow Cytometry : 1-3ug/million cells Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This MPO antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme encoded by the MPO gene and is highly expressed in neutrophils and other cells of the myeloid lineage. MPO is stored within azurophilic granules and plays a key role in innate immune defense by generating reactive oxidant species that contribute to microbial killing. Because of its strong and selective expression in neutrophils and related myeloid cells, MPO has become a widely used marker for identifying granulocytic populations in immunology and hematology research. MPO Antibody for FACS enables sensitive detection of Myeloperoxidase / MPO in single-cell suspensions using flow cytometry, supporting quantitative analysis of immune cell populations in complex biological samples.
Flow cytometry analysis using an MPO Antibody for FACS allows researchers to detect intracellular MPO expression following cell fixation and permeabilization. This approach enables accurate identification of neutrophils, myeloid progenitors, and other MPO-positive leukocytes in peripheral blood, bone marrow, or tissue-derived cell preparations. Because flow cytometry evaluates thousands of individual cells per second, MPO detection by FACS provides rapid and quantitative assessment of myeloid lineage distribution and immune cell composition within heterogeneous samples.
MPO Antibody for FACS is particularly useful for immunophenotyping studies in which myeloid cells must be distinguished from lymphoid populations. When used in multicolor flow cytometry panels, MPO staining can be combined with cell surface markers such as CD45, CD11b, CD15, or other lineage markers to characterize immune cell subsets with high precision. This capability enables detailed analysis of granulocyte differentiation, inflammatory responses, and immune cell recruitment in experimental models or clinical research samples.
Flow cytometry detection of MPO also supports investigation of hematologic diseases, inflammatory disorders, and tumor-associated immune responses. Quantifying MPO-positive cells can help evaluate neutrophil infiltration, bone marrow myelopoiesis, and immune activation states in a wide variety of experimental contexts. Because FACS-based detection provides both quantitative measurements and population-level resolution, MPO Antibody for FACS is a valuable reagent for high-throughput immune profiling and single-cell analysis of myeloid lineage cells.
The stated application concentrations are suggested starting points. Titration of the MPO Antibody for FACS may be required due to differences in protocols and secondary/substrate sensitivity.
Human partial recombinant protein (AA 406-745) was used as the immunogen for this MPO antibody.
After reconstitution, the MPO antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
MPO antibody, Myeloperoxidase antibody, Myeloid peroxidase antibody, Neutrophil myeloperoxidase antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
