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Home >> Antibodies >> MPO Antibody / Myeloperoxidase

MPO Antibody / Myeloperoxidase (R31487)

  Catalog No Formulation Size Price (USD)  
Image R31487 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HL60 cells with MPO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPO antibody.
Western blot testing of human HL60 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
Western blot testing of human 1) HL60 and 2) A431 cell lysate with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
IHC staining of FFPE human spleen tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with MPO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
Gene ID 4353
Localization Nuclear and cytoplasmic
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This MPO antibody is available for research use only.
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Description

Myeloperoxidase is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. MPO is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. Located in the nucleus as well as in the cytoplasm, intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

Application Notes

The stated application concentrations are suggested starting points. Titration of the MPO antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 406-745) was used as the immunogen for this MPO antibody.

Storage

After reconstitution, the MPO antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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