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Home >> Antibodies >> MPI Antibody / Mannose-6 phosphate isomerase

MPI Antibody / Mannose-6 phosphate isomerase [clone 11I4] (RQ5624)

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Image RQ5624 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-2 OS cells with MPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPI antibody.
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Flow cytometry testing of human U-2 OS cells with MPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPI antibody.
Western blot testing of human 1) Caco-2 and 2) HeLa lysate with MPI antibody. Predicted molecular weight ~47 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 11I4
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P34949
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This MPI antibody is available for research use only.
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Description

Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.

Application Notes

Optimal dilution of the MPI antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids A2-K99) was used as the immunogen for the MPI antibody.

Storage

After reconstitution, the MPI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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