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Home >> Antibodies >> MPHOSPH10 Antibody / M phase phosphoprotein 10

MPHOSPH10 Antibody / M phase phosphoprotein 10 (RQ8423)

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  Catalog No Formulation Size Price (USD)  
Image RQ8423 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with MPHOSPH10 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with MPHOSPH10 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HepG2, 3) K562 and 4) HEL cell lysate with MPHOSPH10 antibody. Predicted molecular weight ~79 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with MPHOSPH10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPHOSPH10 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O00566
Localization Nuclear, nucleolar
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This MPHOSPH10 antibody is available for research use only.
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Description

U3 small nucleolar ribonucleoprotein protein MPP10 is a protein that in humans is encoded by the MPHOSPH10 gene. This gene encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing.

Application Notes

Optimal dilution of the MPHOSPH10 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (L15-A661) was used as the immunogen for the MPHOSPH10 antibody.

Storage

After reconstitution, the MPHOSPH10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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