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Home >> Antibodies >> Monomethyl-Histone H4 Antibody / Lysine 5 / H4K5me1

Monomethyl-Histone H4 Antibody / Lysine 5 / H4K5me1 [clone RM457] (R20471)

  Catalog No Formulation Size Price (USD)  
Image R20471-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Microvalidated Recrabbitmono
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Western blot testing of recombinant Histone H4 (1) and acid extracts of human HeLa cells using recombinant Monomethyl-Histone H4 antibody at 0.2 ug/ml.
This recombinant Monomethyl-Histone H4 antibody specifically reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in Histone H4.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM457
Purity Protein A purified from animal origin-free supernatant
UniProt P62805
Applications Western blot : 0.1ug/ml-2ug/ml
ELISA : 0.5ug/ml-1ug/ml
Limitations This recombinant Monomethyl-Histone H4 antibody is available for research use only.
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Description

This antibody reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in Histone H4.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Monomethyl-Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A monomethyl-lysine 5 peptide corresponding to Histone H4 was used as the immunogen for the Monomethyl-Histone H4 antibody.

Storage

Store the recombinant Monomethyl-Histone H4 antibody at -20oC.

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