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Home >> Antibodies >> Monofunctional C1-tetrahydrofolate synthase Antibody / MTHFD1L

Monofunctional C1-tetrahydrofolate synthase Antibody / MTHFD1L (RQ6778)

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  Catalog No Formulation Size Price (USD)  
Image RQ6778 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with Monofunctional C1-tetrahydrofolate synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with Monofunctional C1-tetrahydrofolate synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human K562, 3) rat brain and 4) mouse brain tissue lysate with Monofunctional C1-tetrahydrofolate synthase antibody. Predicted molecular weight ~106 kDa.
Flow cytometry testing of human HEL cells with Monofunctional C1-tetrahydrofolate synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Monofunctional C1-tetrahydrofolate synthase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6UB35
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Monofunctional C1-tetrahydrofolate synthase antibody is available for research use only.
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Description

Monofunctional C1-tetrahydrofolate synthase, mitochondrial also known as formyltetrahydrofolate synthetase, is an enzyme that in humans is encoded by the MTHFD1L gene (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like). The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Monofunctional C1-tetrahydrofolate synthase antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R43-V833) was used as the immunogen for the Monofunctional C1-tetrahydrofolate synthase antibody.

Storage

After reconstitution, the Monofunctional C1-tetrahydrofolate synthase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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