- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
MOAT-B Antibody / Multidrug Resistance Transporter Antibody [clone ABCC4/9178] (V5644)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5644-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V5644-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V5644SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | ABCC4/9178 |
| Purity | Protein G affinity |
| UniProt | O15439 |
| Localization | Membrane |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml Western Blot : 2-4ug/ml |
| Limitations | This MOAT-B antibody is available for research use only. |
|
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Multidrug resistance-associated protein 4 (ABCC4), also known as MOAT-B and MRP4, is a member of the ATP-binding cassette (ABC) transporter family that mediates ATP-dependent efflux of a wide range of endogenous signaling molecules and xenobiotic compounds. ABCC4 is primarily localized to the plasma membrane, where it functions as an active transporter regulating intracellular concentrations of cyclic nucleotides, prostaglandins, and therapeutic agents. The MOAT-B Antibody is designed to detect this membrane-associated transporter, supporting studies of drug transport, multidrug resistance, and signaling regulation.
MOAT-B antibody, also referred to as ABCC4 antibody and MRP4 antibody in the literature, recognizes a broadly expressed transporter protein found in tissues including kidney, liver, brain, and hematopoietic compartments. ABCC4 is particularly enriched in epithelial barrier tissues where directional transport is required, such as renal tubular epithelium and hepatobiliary systems. Western blot analysis typically detects ABCC4 as a high-molecular-weight band above 130 kDa, consistent with its predicted size and glycosylated membrane protein structure, although migration may vary depending on sample preparation and glycosylation status.
Structurally, ABCC4 contains two transmembrane domains and two cytosolic nucleotide-binding domains characteristic of ABC transporters, enabling ATP binding and hydrolysis to drive substrate transport across cellular membranes. The protein undergoes glycosylation, which contributes to its apparent molecular weight and influences electrophoretic mobility. Its membrane localization and transporter architecture distinguish it from intracellular metabolic enzymes and reflect its role in active substrate export.
Functionally, ABCC4 plays a central role in regulating intracellular signaling pathways through the export of cyclic nucleotides such as cAMP and cGMP, as well as lipid mediators including prostaglandins. By controlling the extracellular availability of these molecules, ABCC4 contributes to processes such as inflammation, vascular tone regulation, and platelet activation. In epithelial tissues, it supports directional transport and contributes to maintenance of tissue homeostasis.
ABCC4 is also a key mediator of multidrug resistance in cancer, where its expression can reduce intracellular accumulation of chemotherapeutic agents, contributing to decreased drug sensitivity. Its activity has been linked to resistance against antiviral, anticancer, and anti-inflammatory drugs, making it an important target in pharmacology and cancer research. Expression of ABCC4 in tumor cells and surrounding tissue compartments highlights its role in shaping drug response and tumor microenvironment signaling.
Beyond oncology, ABCC4 contributes to immune regulation and physiological signaling by modulating extracellular concentrations of signaling molecules in diverse tissues. Its broad substrate specificity and tissue distribution underscore its importance in both normal physiology and disease states. Clone ABCC4/9178 is a mouse monoclonal antibody designed to detect MOAT-B (ABCC4) with high specificity. Its ability to identify this transporter in cell and tissue samples supports research into drug transport mechanisms, cellular signaling, and multidrug resistance biology.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the MOAT-B antibody should be determined by the researcher.
A portion of amino acids 1-200 from human ABCC4 protein was used as the immunogen for the MOAT-B antibody.
Aliquot the MOAT-B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
MOAT-B antibody, ABCC4 antibody, MRP4 antibody, Multidrug resistance protein 4 antibody, ABCC4 transporter antibody
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