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Home >> Antibodies >> MMUT Antibody / Methylmalonyl Coenzyme A Mutase

MMUT Antibody / Methylmalonyl Coenzyme A Mutase [clone 2D6] (RQ5944)

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  Catalog No Formulation Size Price (USD)  
Image RQ5944 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) K562, 3) HEK293, 4) PC-3, 5) Caco-2, 6) Raji, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with MMUT antibody. Predicted molecular weight ~83 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 2D6
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P22033
Localization Cytoplasmic (mitochondria)
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Limitations This MMUT antibody is available for research use only.
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Description

Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.

Application Notes

Optimal dilution of the MMUT antibody should be determined by the researcher.

Immunogen

Amino acids LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE from the human protein were used as the immunogen for the MMUT antibody.

Storage

After reconstitution, the MMUT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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