MMP20 Antibody / Matrix metalloproteinase 20 (FY13191)

Western blot analysis of MMP20 using anti-MMP20 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP20 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of MMP20 shows a predominant band just below the 50 kDa marker, consistent with a processed form of the ~54 kDa zymogen, plus additional species at ~35 kDa, ~60 kDa, and ~105 kDa that likely reflect autolytic fragments, variably glycosylated intermediates, and higher-order complexes, respectively.