MIRO1 Antibody / RHOT1 Polyclonal Antibody (FY13689)

MIRO1 Antibody IF Staining of Human T47D Cells. Immunofluorescence analysis of human T47D cells using RHOT1 Polyclonal Antibody at 5 ug/ml. Green fluorescence demonstrates predominantly cytoplasmic immunoreactivity with a punctate and granular distribution, consistent with the mitochondrial association of MIRO1. DAPI counterstaining identifies nuclei in blue. Enzyme-mediated antigen retrieval was performed for 15 minutes, followed by overnight primary antibody incubation at 4oC and detection with a DyLight 488-conjugated secondary antibody.

MIRO1 Antibody IHC Staining of Human Lung Cancer Tissue. Immunohistochemical analysis of FFPE human lung cancer tissue using RHOT1 Polyclonal Antibody at 2 ug/ml. Brown DAB staining is visible in tumor cells forming glandular structures, with cytoplasmic immunoreactivity and additional staining in portions of the surrounding tissue. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8, followed by overnight primary antibody incubation at 4oC.

MIRO1 Antibody IHC Staining of Human Laryngeal Squamous Cell Carcinoma Tissue. Immunohistochemical analysis of FFPE human laryngeal squamous cell carcinoma tissue using RHOT1 Polyclonal Antibody at 2 ug/ml. Brown DAB staining is distributed throughout the tumor cell population, with predominantly cytoplasmic immunoreactivity against the blue hematoxylin counterstain. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8, followed by overnight primary antibody incubation at 4oC.

MIRO1 Antibody IHC Staining of Human Gallbladder Adenosquamous Carcinoma Tissue. Immunohistochemical analysis of FFPE human gallbladder adenosquamous carcinoma tissue using RHOT1 Polyclonal Antibody at 2 ug/ml. Brown DAB staining is visible in clusters of tumor cells, with cytoplasmic immunoreactivity and additional staining in scattered cells throughout the surrounding tissue. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8, followed by overnight primary antibody incubation at 4oC.

MIRO1 Antibody IHC Staining of Human Thyroid Papillary Carcinoma Tissue. Immunohistochemical analysis of FFPE human thyroid papillary carcinoma tissue using RHOT1 Polyclonal Antibody at 2 ug/ml. Brown DAB staining is prominent in tumor cells, with predominantly granular cytoplasmic immunoreactivity and variable peripheral cellular staining against the blue hematoxylin counterstain. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8, followed by overnight primary antibody incubation at 4oC.

MIRO1 Antibody IHC Staining of Human Cerebellum Tissue. Immunohistochemical analysis of FFPE human cerebellum tissue using RHOT1 Polyclonal Antibody at 2 ug/ml. Brown DAB staining is visible in a large neuronal cell body near the Purkinje cell layer, with predominantly cytoplasmic immunoreactivity against the blue hematoxylin counterstain. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8, followed by overnight primary antibody incubation at 4oC.

MIRO1 Antibody Western Blot Analysis of HepG2, THP-1, Rat Liver, Mouse Heart and Mouse Liver Lysates. Western blot analysis using RHOT1 Polyclonal Antibody at 0.25 ug/ml. Lane 1: human HepG2 cells; lane 2: human THP-1 cells; lane 3: rat liver; lane 4: mouse heart; lane 5: mouse liver. Each lane contains 30 ug of total protein under reducing conditions. Immunoreactive bands are detected at approximately 71 kDa across all five samples, consistent with the calculated molecular weight of MIRO1/RHOT1 at approximately 70.8 kDa.

MIRO1 Antibody Western Blot Validation in HeLa Wild-Type and RHOT1 Knockout Cells. Western blot analysis of human HeLa wild-type (WT, lane 1) and RHOT1 knockout (KO, lane 2) whole-cell lysates using RHOT1 Polyclonal Antibody at 0.5 ug/ml. Each lane contains 30 ug of total protein under reducing conditions. An immunoreactive band at approximately 71 kDa is detected in both samples, with reduced signal in the KO lysate. The observed molecular weight is consistent with the calculated size of approximately 70.8 kDa. GAPDH serves as the loading control.