MIEF1 Antibody / Mitochondrial dynamics protein MID51 (RQ6428)

IHC staining of FFPE human renal clear cell carcinoma tissue with MIEF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human rectal cancer tissue with MIEF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with MIEF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HT1080, 2) Jurkat, 3) K562, 4) HL60, 5) HEK293, 6) U937, 7) HepG2, 8) rat heart, 9) rat kidney and 10) mouse kidney tissue lysate with MIEF1 antibody. Predicted molecular weight ~51 kDa.

Flow cytometry testing of human SiHa cells with MIEF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIEF1 antibody.

Immunoprecipitation and western blot analysis of MIEF1 expression. Human K562 whole cell lysates were used for immunoprecipitation with anti-MIEF1 antibody. Lane 1 shows input K562 whole cell lysate (30 ug). Lane 2 shows immunoprecipitation performed using rabbit control IgG instead of the primary antibody, serving as a negative control. Lane 3 shows immunoprecipitation using anti-MIEF1 antibody (2 ug) incubated with K562 whole cell lysate (500 ug). Immunoprecipitated proteins were resolved by SDS-PAGE, transferred to membrane, and detected using anti-MIEF1 antibody followed by an HRP-conjugated goat anti-rabbit IgG light chain secondary antibody. A specific band corresponding to MIEF1 is detected at the predicted molecular weight of approximately 51 kDa, consistent with mitochondrial elongation factor 1.