MICA Antibody / Human Tissue-Enriched Detection Antibody [clone MICA/4443] (V9703)

MICA Antibody Human Stomach IHC. Immunohistochemistry staining of FFPE human stomach with MICA antibody (clone MICA/4443). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MICA Antibody Human Kidney IHC. Immunohistochemistry staining of FFPE human kidney tissue with MICA antibody (clone MICA/4443). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MICA Antibody Protein Microarray Specificity Analysis. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MICA antibody (clone MICA/4443). These results demonstrate the foremost specificity of the MICA/4443 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

MICA Antibody Human Lung WB. Western blot analysis of MHC class I polypeptide-related sequence A (MICA) expression in multi-species lung tissue lysates using MICA antibody clone MICA/4443. Lane 1: human lung lysate, Lane 2: mouse lung lysate, Lane 3: rat lung lysate, Lane 4: hamster lung lysate, Lane 5: guinea pig lung lysate. A distinct band is detected at approximately 50-60 kDa in human lung lysate, consistent with glycosylated forms of MICA. Minimal or no detectable signal is observed in the non-human lanes under these conditions, supporting a human tissue-enriched detection profile for this clone.

MICA Antibody Kidney Tissue WB. Western blot analysis of MHC class I polypeptide-related sequence A (MICA) expression in human kidney tissue lysate using MICA antibody clone MICA/4443. Lane 1: human kidney lysate. A broad band is detected at approximately 70-90 kDa, consistent with heavily glycosylated forms of MICA. The higher apparent molecular weight and band heterogeneity reflect tissue-specific glycosylation and post-translational modification commonly observed in epithelial-rich tissues such as kidney, supporting a human tissue-enriched detection profile for this clone.

SDS-PAGE analysis of purified, BSA-free MICA antibody (MICA/4443) as confirmation of integrity and purity.