MICA Antibody / Cross-Species Reactivity Antibody [clone MICA/4442] (V9702)

MICA Antibody Stomach Tissue IHC. Immunohistochemistry staining of FFPE human stomach with MICA antibody (clone MICA/4442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

MICA Antibody Kidney Tissue IHC. Immunohistochemistry staining of FFPE human kidney tissue with MICA antibody (clone MICA/4442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MICA antibody (clone MICA/4442) as confirmation of integrity and purity.

MICA Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MICA antibody (clone MICA/4442). These results demonstrate the foremost specificity of the MICA/4442 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

MICA Antibody Lung Tissue WB. Western blot analysis of MHC class I polypeptide-related sequence A (MICA) expression across multi-species lung tissue lysates using MICA antibody clone MICA/4442. Lane 1: human lung lysate, Lane 2: mouse lung lysate, Lane 3: rat lung lysate, Lane 4: hamster lung lysate, Lane 5: guinea pig lung lysate. A band is detected at approximately 50-65 kDa, consistent with glycosylated forms of MICA. The consistent detection across species supports the use of this antibody for cross-species reactivity applications, reflecting conserved expression and post-translational processing of MICA in lung tissue.

MICA Antibody Kidney Tissue WB. Western blot analysis of MHC class I polypeptide-related sequence A (MICA) expression in human kidney tissue lysate using MICA antibody clone MICA/4442. Lane 1: human kidney lysate. A broad band is detected at approximately 70-90 kDa, consistent with heavily glycosylated forms of MICA. The higher apparent molecular weight and band heterogeneity reflect tissue-specific glycosylation and post-translational modification commonly observed for MICA in epithelial-rich tissues such as kidney.