MICA Antibody (F51246)

Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: Daudi ; 3: A431; 4: MCF-7; 5: U-87MG; 6: THP-1; Predicted molecular weight: ~43 kDa but may be observed at 38-62 kDa depending on truncation and glycosylation level.

Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: U-87 MG lysate; 2: A431; 3: Jurkat; Predicted molecular weight: ~43kDa but may be observed at 38-62kDa depending on truncation and glycosylation level.

Western blot testing of MICA antibody at 1:1000 dilution + HeLa lysate

Flow cytometric analysis of SK-BR-3 cells using MICA antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary.

Immunofluorescent staining of fixed and permeabilized human HeLa cells with MICA antibody (green), phalloidin (red, cytoplasmic actin) and DAPI (blue, nuclear).

Immunofluorescent staining of fixed and permeabilized human HeLa cells with MICA antibody (green), phalloidin (red, cytoplasmic actin) and DAPI (blue, nuclear).

IHC staining of FFPE human skin with MICA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.