Methyl-CpG-binding protein 2 Antibody / MECP2 (FY13459)

Immunohistochemistry analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in mouse brain tissue, with prominent nuclear staining observed in neuronal cells throughout the cortex. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with MECP2 antibody overnight at 4°C. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Immunohistochemistry analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in rat brain tissue, showing nuclear localization in neuronal cell populations. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with MECP2 antibody overnight at 4°C. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Immunofluorescence analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in mouse brain tissue, with strong nuclear fluorescence observed in neuronal cells. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with MECP2 antibody (red) overnight at 4°C. Immunoreactivity was visualized using fluorescence detection.

Immunofluorescence analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in mouse brain tissue, with strong nuclear fluorescence observed in neuronal cells. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with MECP2 antibody (red) overnight at 4°C. Immunoreactivity was visualized using fluorescence detection.

Immunofluorescence analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in rat brain tissue, showing nuclear localization within neuronal populations. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with MECP2 antibody (red) overnight at 4°C. Immunoreactivity was visualized using fluorescence detection.

Immunofluorescence analysis of MECP2 using Methyl-CpG-binding protein 2 antibody and alpha tubulin antibody. MECP2 expression was examined in human U2OS cells, with MECP2 signal observed in the nucleus and alpha tubulin outlining the cytoplasmic microtubule network. Cells were blocked with normal goat serum and incubated with MECP2 antibody (green) and alpha tubulin antibody (red) overnight at 4°C. Immunoreactivity was visualized using fluorescence detection.

Western blot analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in human U251 (lane 1), K562 (lane 2), U2OS (lane 3), and HEK293T (lane 4) whole cell lysates, as well as rat brain tissue (lane 5), rat C6 cells (lane 6), mouse brain tissue (lane 7), and mouse Neuro-2a cells (lane 8). A predominant band is detected at approximately 75 kDa, migrating above the predicted molecular weight of approximately 52 kDa based on the MECP2 amino acid sequence. This upward shift is consistent with the well-documented anomalous SDS-PAGE migration of MECP2, which is attributed to its high content of intrinsically disordered regions, basic residues, and extensive post-translational modifications such as phosphorylation, all of which are known to increase the apparent molecular weight of chromatin-associated proteins.

Flow cytometry analysis of MECP2 using Methyl-CpG-binding protein 2 antibody. MECP2 expression was examined in human HEK293T cells following fixation with 4% paraformaldehyde and permeabilization to enable intracellular staining. Cells were incubated with MECP2 antibody and detected using a fluorescent secondary antibody (blue). An isotype control stained under identical conditions is shown in green, and an unstained control is shown in red. The rightward fluorescence shift observed with MECP2 antibody staining indicates specific intracellular detection of MECP2.