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Home >> Antibodies >> Mesothelin Antibody

Mesothelin Antibody (R31700)

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  Catalog No Formulation Size Price (USD)  
Image R31700 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of Mesothelin antibody and recombinant human protein (0.5ng)
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Western blot testing of Mesothelin antibody and recombinant human protein (0.5ng)
IHC-P: Mesothelin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
Flow cytometry testing of human SiHa cells with Mesothelin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Mesothelin antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 10232
Localization Cytoplasmic, cell surface, secreted
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA free format for coating
Limitations This Mesothelin antibody is available for research use only.
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Description

Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, Megakaryocyte potentiating factor and Mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Mesothelin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 306-576) was used as the immunogen for this Mesothelin antibody.

Storage

After reconstitution, the Mesothelin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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