Mesothelin Antibody / Microarray Specificity Validated Antibody [clone MSLN/3385] (V8689)

Mesothelin Antibody Mesothelioma IHC. Immunohistochemistry analysis of mesothelin expression in FFPE human mesothelioma using clone MSLN/3385. Tumor cells demonstrate membranous HRP-DAB brown staining with focal cytoplasmic signal, consistent with mesothelin surface localization. The staining pattern highlights mesothelial tumor cell populations while maintaining low background in surrounding stromal elements. The clear and selective signal is consistent with microarray specificity validated performance, supporting high-confidence detection of mesothelin in tissue sections. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

Mesothelin Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using clone MSLN/3385 demonstrates highly selective binding to mesothelin (MSLN). The antibody shows a dominant signal for MSLN with a strong Z-score and clear separation from all other proteins, indicating exceptional specificity and minimal off-target reactivity.
The Z-score reflects the signal intensity for each protein relative to the overall array, expressed as standard deviations above the mean. The S-score represents the difference between adjacent ranked Z-scores and provides a measure of target specificity. The large separation between MSLN and secondary targets supports high-confidence identification of mesothelin in complex biological samples.

SDS-PAGE analysis of purified, BSA-free Mesothelin antibody (clone MSLN/3385) as confirmation of integrity and purity.

Western blot testing of human OVCAR-3 cell lysate with specificity validated Mesothelin antibody. Predicted molecular weight ~70 kDa (precursor), ~40 kDa (processed form).