Mesothelin Antibody / Tumor Marker Antibody [clone MSLN/2131] (V3820)

Mesothelin Antibody Lung Mesothelioma IHC. Immunohistochemistry analysis of mesothelin expression in FFPE human lung mesothelioma using clone MSLN/2131. Strong membranous HRP-DAB brown staining is observed across tumor cells with accompanying cytoplasmic signal, consistent with mesothelin cell surface localization and processing. The staining pattern highlights cohesive tumor cell architecture and clearly distinguishes mesothelioma cells from surrounding stromal components, supporting its use as a tumor marker in tissue sections. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

Mesothelin Antibody Endometrial Carcinoma IHC. Immunohistochemistry analysis of mesothelin expression in FFPE human endometrial carcinoma using clone MSLN/2131. Tumor epithelial cells demonstrate membranous HRP-DAB brown staining with focal cytoplasmic signal, consistent with mesothelin surface localization and processing. Staining highlights glandular tumor structures, while surrounding stromal components show minimal reactivity, supporting its role as a tumor marker in epithelial malignancies. Heat-induced epitope retrieval was performed using pH 9 Tris-EDTA buffer.

IHC testing of FFPE human tonsil with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE mouse lung with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat stomach with Mesothelin antibody (clone MSLN/2131). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Mesothelin antibody (clone MSLN/2131). These results demonstrate the foremost specificity of the MSLN/2131 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Mesothelin antibody (clone MSLN/2131) as confirmation of integrity and purity.

Mesothelin Antibody OVCAR-3 Cancer Cell WB. Western blot analysis of human OVCAR-3 cell lysate using Mesothelin Antibody. A prominent band is detected at approximately 40-50 kDa, consistent with the processed form of mesothelin generated from cleavage of the ~70 kDa precursor protein. Mesothelin undergoes proteolytic processing to yield a membrane-associated fragment, explaining the lower apparent molecular weight observed. This banding pattern aligns with reported mesothelin expression in ovarian cancer-derived cell lines.