• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> MERTK Polyclonal Antibody / Mer Tyrosine Kinase Antibody

MERTK Polyclonal Antibody / Mer Tyrosine Kinase Antibody (RQ6497)

  Catalog No Formulation Size Price (USD)  
Image RQ6497 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
Bulk quote request
MERTK Polyclonal Antibody in Human Rectal Cancer IHC. Immunohistochemistry of FFPE human rectal cancer sections using MERTK Polyclonal Antibody. The tissue shows distinct brown immunoreactivity within the carcinoma, demonstrating localization of MERTK in malignant colorectal tissue. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. This tissue validation supports detection of Mer receptor expression using the Mer Tyrosine Kinase Antibody.
MERTK Polyclonal Antibody in Human Gallbladder Adenocarcinoma IHC. Immunohistochemistry of FFPE human gallbladder adenocarcinoma sections using MERTK Polyclonal Antibody. Brown immunoreactivity is visible within the malignant tissue, demonstrating MERTK detection in gallbladder adenocarcinoma. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. The staining provides additional tumor-tissue validation for this Mer Tyrosine Kinase Antibody
MERTK Polyclonal Antibody in Human Liver Cancer IHC. Immunohistochemistry of FFPE human liver cancer sections using MERTK Polyclonal Antibody. Distinct brown staining is present within the tumor tissue against the hematoxylin-counterstained background, demonstrating detection of MERTK in malignant hepatic tissue. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. The result expands the tissue validation available for the Mer Tyrosine Kinase Antibody.
MERTK Polyclonal Antibody in Human Lung Cancer IHC. Immunohistochemistry of FFPE human lung cancer sections using MERTK Polyclonal Antibody. Brown immunoreactivity is evident within the malignant tissue, providing visualization of MERTK expression in the lung cancer specimen. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. This staining further demonstrates tissue-based detection with the Mer Tyrosine Kinase Antibody.
MERTK Polyclonal Antibody in Human Lymphoma IHC. Immunohistochemistry of FFPE human lymphoma sections using MERTK Polyclonal Antibody. The image demonstrates brown immunoreactivity within the lymphoma tissue, supporting detection of MERTK in a hematologic malignancy context. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. The result complements the solid-tumor IHC validation available for this Mer Tyrosine Kinase Antibody.
MERTK Polyclonal Antibody in Human Placenta IHC. Immunohistochemistry of FFPE human placental sections using MERTK Polyclonal Antibody. Brown staining is visible within placental structures, demonstrating MERTK localization in non-neoplastic human tissue. HIER was performed by boiling sections in pH 8 EDTA for 20 min and allowing them to cool before testing. This result provides a normal-tissue counterpart to the tumor IHC validation for the Mer Tyrosine Kinase Antibody.
MERTK Polyclonal Antibody Multi-Species Western Blot. Western blot analysis of human HepG2 (lane 1), human Jurkat (lane 2), human Caco-2 (lane 3), rat testis (lane 4), rat lung (lane 5), mouse testis (lane 6) and mouse lung (lane 7) lysates using MERTK Polyclonal Antibody. MERTK has a predicted polypeptide mass near 110 kDa but can migrate substantially higher, approximately 110-205 kDa, because of extensive glycosylation of the extracellular receptor domain. Detection across human, rat and mouse samples demonstrates the broad species utility of this Mer Tyrosine Kinase Antibody.
MERTK Polyclonal Antibody in HepG2 Cells by FACS. Flow cytometry analysis of human HepG2 cells using MERTK Polyclonal Antibody at 1 ug per million cells following blocking with goat serum. Cells alone are shown in red, the isotype control in green and MERTK antibody staining in blue. The blue population shows a clear rightward fluorescence shift relative to the controls, demonstrating detection of cell-associated MERTK. This flow cytometry validation complements the tissue and western blot data available for the Mer Tyrosine Kinase Antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q12866
RRID AB_3756741
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This MERTK Polyclonal Antibody / Mer Tyrosine Kinase Antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : IHC-P
    Reactivity : Human
  • Applications : WB, FACS
    Reactivity : Human, Mouse, Rat, Monkey
  • Applications : IHC, FACS, IF, WB, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Mouse, Rat
  • Applications : WB, ELISA (peptide)
    Reactivity : Human

Description

MERTK Polyclonal Antibody / Mer Tyrosine Kinase Antibody recognizes MER proto-oncogene tyrosine kinase, a member of the TAM receptor tyrosine kinase family together with AXL and TYRO3. MERTK is also known as MerTK, MER, c-Mer and Eyk. The receptor participates in cellular signaling pathways that regulate phagocytosis, survival, migration and immune homeostasis and is particularly important for the recognition and clearance of apoptotic cells.

MERTK is a single-pass transmembrane protein containing two extracellular immunoglobulin-like domains, two fibronectin type III domains and an intracellular tyrosine kinase domain. Its extracellular region interacts with the ligands GAS6 and Protein S, which can bridge the receptor to phosphatidylserine exposed on apoptotic cells. Receptor activation promotes intracellular signaling that facilitates engulfment of apoptotic material through efferocytosis while helping regulate inflammatory responses associated with dying cells.

MERTK function is particularly important in macrophages and other professional phagocytes. Clearance of apoptotic cells helps maintain tissue homeostasis and prevents the accumulation of cellular debris that could otherwise promote inflammation. Mer Tyrosine Kinase Antibody reagents are therefore useful for investigating the relationship between TAM receptor signaling, phagocyte biology and regulation of innate immune responses.

MERTK also has an important role in retinal pigment epithelial cells, where it participates in phagocytic removal of shed photoreceptor outer segments. Loss-of-function mutations in the MERTK gene can disrupt this process and are associated with inherited retinal degeneration, including forms of retinitis pigmentosa. Aberrant MERTK expression and signaling are additionally investigated in cancer, where receptor activity can influence tumor cell survival and the immune characteristics of the tumor microenvironment.

NSJ Bioreagents offers this affinity-purified rabbit polyclonal reagent for research into MERTK expression and receptor biology. The MERTK Polyclonal Antibody terminology emphasizes the official gene symbol and polyclonal format, while Mer Tyrosine Kinase Antibody provides an expanded target-name search term.

A MERTK Polyclonal Antibody provides a reagent for investigating MERTK expression and TAM-family biology, while a Mer Tyrosine Kinase Antibody supports research into efferocytosis, immune regulation and receptor tyrosine kinase signaling.

For a Protein Microarray Validated monoclonal antibody to this target, see our MerTK Antibody MERTK/3015 / MERTK Monoclonal Antibody.

Application Notes

Optimal dilution of the MERTK Polyclonal Antibody / Mer Tyrosine Kinase Antibody should be determined by the researcher.

Immunogen

An E. coli-derived human protein (amino acids A21-A489) was used as the immunogen for the MERTK Polyclonal Antibody.

Storage

After reconstitution, the MERTK Polyclonal Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Mer Tyrosine Kinase antibody, MerTK antibody, MER antibody, c-Mer antibody, Mer Receptor Tyrosine Kinase antibody, MER Proto-Oncogene Tyrosine Kinase antibody, Eyk antibody

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.