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Home >> Antibodies >> MENA Antibody / ENAH

MENA Antibody / ENAH (RQ6766)

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  Catalog No Formulation Size Price (USD)  
Image RQ6766 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human liver cancer tissue with MENA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with MENA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE metaplasia of squamous cells of the renal pelvis tissue with MENA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with MENA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with MENA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human MCF7, 2) human A431, 3) human T-47D, 4) human SiHa, 5) rat stomach, 6) mouse stomach and 7) mouse pancreas tissue lysate with MENA antibody. Predicted molecular weight ~66 kDa but can be observed at ~90 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q8N8S7
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This MENA antibody is available for research use only.
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Description

Protein enabled homolog, also called MENA, is a protein that in humans is encoded by the ENAH gene. This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3.

Application Notes

Optimal dilution of the MENA antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids F32-E551) was used as the immunogen for the MENA antibody.

Storage

After reconstitution, the MENA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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