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Home >> Antibodies >> Melanoma Marker Antibody Cocktail (MART-1 & gp100)

Melanoma Marker Antibody Cocktail (MART-1 & gp100) [clone DT101+ BC199 + HMB45] (V8320)

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  Catalog No Formulation Size Price (USD)  
Image V8320-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8320-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8320SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa + Mouse IgG1, kappa
Clone Name DT101+ BC199 + HMB45
Purity Protein G affinity chromatography
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Melanoma Marker antibody cocktail is available for research use only.
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Description

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Application Notes

Optimal dilution of the Melanoma Marker antibody cocktail should be determined by the researcher.

Immunogen

Recombinant hMART-1 protein (DT101 & BC199) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogen for this Melanoma Marker antibody cocktail.

Storage

Store the Melanoma Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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