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Home >> Antibodies >> Melanoma Marker Antibody Cocktail

Melanoma Marker Antibody Cocktail [clone A103 + HMB45 + T311] (V7396)

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  Catalog No Formulation Size Price (USD)  
Image V7396-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7396-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7396SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7396IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human melanoma with Melanoma Marker antibody cocktail (A103 + T311 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human melanoma with Melanoma Marker antibody cocktail (A103 + T311 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human COLO-38 cell lysate with Melanoma Marker antibody cocktail (clones A103 + HMB45 + T311).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1/IgG2a
Clone Name A103 + HMB45 + T311
Purity Protein G affinity chromatography
UniProt Q16655 (Melan-A) , P14679 (Tyrosinase) , P40967 (gp100)
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This Melanoma Marker antibody cocktail is available for research use only.
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Description

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Application Notes

The optimal dilution of the Melanoma Marker antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant MART-1 protein (A103), recombinant tyrosinase protein (T311) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogens for this Melanoma Marker antibody cocktail.

Storage

Store the Melanoma Cell Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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