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Home >> Antibodies >> Melan-A Antibody

Melan-A Antibody [clone MRT3V] (V7016)

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  Catalog No Formulation Size Price (USD)  
Image V7016-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7016-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7016SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7016IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A antibody (clione MRT3V). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA, for 10-20 min followed by cooling at RT for 20 min.
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A antibody (clione MRT3V). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MRT3V
Purity Protein G affinity chromatography
UniProt Q16655
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Melan-A antibody is available for research use only.
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Description

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Application Notes

Optimal dilution of the Melan-A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the Melan-A antibody.

Storage

Store the Melan-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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